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❶Characteristics of a hamster cell clone transformed by polyoma virus. By passage 17the Y chromosome can no longer be detected by aav amelogenin analysis. A classic example of random X-inactivation is presented by the calico, or tortoiseshell, cat. Nardone RM. Eradication of cross-contaminated cell lines: a call for action.

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Do you know the sex of your cells? Not a question that is frequently heard around the lab bench, yet thanks to recent research is probably one that should be asked.

It is self-evident that cervical epithelial cells would be derived from female tissue and prostate cells from a male subject exemplified by HeLa and LnCaP, respectivelyyet beyond these obvious examples, it would be true to say that the sex of cell lines derived from non-reproductive tissue, such as lung, intestine, kidney, for example, is given minimal if any thought.

After all, what possible impact could the presence of a Y chromosome have on the biochemistry and cell biology of tissues such as the exocrine pancreatic acini? Intriguingly, recent evidence has suggested that far from being irrelevant, genes Sex sm Darlington av on the sex chromosomes can have a marked impact on the biology of such diverse tissues as neurons and renal cells. In this review we discuss recent data arguing that the sex of cells being used in experiments can impact the cell's biology, and Male revues in Gateshead provide a table outlining the sex of cell lines that have appeared in AJP-Cell Physiology over the past decade.

Over a decade later, the recommendations of this report Sex sm Darlington av received meager acceptance. Most researchers acknowledge the importance of describing the sex of animals used in studies. Sex selection is obviously important in some studies.

For example, it is obvious that a research study on milk production and lactation would utilize only female animals, whereas studies on spermatogenesis would be confined to male subjects.

Despite the clear importance of knowing the sex when using whole animals, such sex assignments are paid scant attention when studies are performed using cell lines Fig. After all, cells derived from male and female organisms display the same general characteristics. Cells derived from both sexes Sex sm Darlington av metabolic processes, proliferate, and undergo differentiation. Cells, whether they are obtained from a male or female, possess a nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus, and other cellular organelles.

The assumption is made that, because there is really no difference in architecture or function between cells from male and female organisms, the Instructions to Authors when submitting to the AJP-Cell Physiologywhich state that the source of all cells utilized species, sex. A survey of a recent issue of AJP-Cell Physiology revealed that only two articles referenced the sex of the animal used, and none referenced the sex of the cell lines employed.

A recent review of publications describing the use of cultured cells in cardiovascular studies found a similar paucity of information on the sex of the cell lines utilized Why is the sex of Orchard hotel Rochdale girl friendly lines Darlintton in studies so often omitted from the final published article? It is likely that the sex of the cells being used was simply not known by the investigators, who, like most of us, simply regard the sex of our cells as irrelevant.

The utility of cultured cells in identifying biological mechanisms, pathways, and processes is beyond doubt. Indeed, the results from such studies are often the basis for the development of new diagnostic and therapeutic interventions in human medicine. However, only half of the population may have a sex the same as the cell line on which the diagnostic test or treatment Sex sm Darlington av developed. In this review, we provide a setting for the Darllington of differences between male Sex sm Darlington av female cells and highlight why these differences will likely provide novel insights into the roles of the X and Y chromosomes.

Information on the sex of cell lines routinely used by authors of publications in AJP-Cell Physiology is also presented. Finally, we pose several questions that we hope will guide Massage pendleton Salford scientific community with regard to the potential role of sex in studies using cell lines and at least cause researchers to consider the impact of the sex of a cell on the interpretation of experimental results.